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Ohorts. Clin Infect Dis 2012, 54:167?74. 34. Hospenthal DR, Murray CK, Andersen RC, et
BMC Infectious Diseases 2013, 13:69 http://www.biomedcentral.com/1471-2334/13/RESEARCH ARTICLEOpen Access153559-49-0 differences in fungi present in induced sputum 163521-12-8 chemical information samples from asthma patients and non-atopic PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28666375 controls: a community based case control studyHugo Cornelis van Woerden1*, Clive Gregory1*, Richard Brown2, Julian Roberto Marchesi2, Bastiaan Hoogendoorn1 and Ian Price MatthewsAbstractBackground: There is emerging evidence for the presence of an extensive microbiota in human lungs. Methods: Induced sputum samples were collected in a case control study of asthma patients and control subjects drawn from the community in Wandsworth, London. Samples from both groups were pooled and then tested for eukaryotes. DNA was amplified using standard PCR techniques, followed by pyrosequencing and comparison of reads to databases of known sequences to determine in a semi-quantitative way the percentage of DNA from known species in each of the two pooled samples. Results: A total of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28534427 136 fungal species were identified in the induced sputum samples, with 90 species more common in asthma patients and 46 species more common in control subjects. Psathyrella candolleana, Malassezia pachydermatis, Termitomyces clypeatus and Grifola sordulenta showed a higher percentage of reads in the sputum of asthma patients and Eremothecium sinecaudum, Systenostrema alba, Cladosporium cladosporioides and Vanderwaltozyma CS-0654 web polyspora showed a higher percentage of reads in the sputum of control subjects. A statistically significant difference in the pattern of fungi that were present in the respective samples was demonstrated using the Phylogenetic (P) test (P < 0.0001). Conclusion: This study is novel in providing evidence for the widespread nature of fungi in the sputum of healthy and asthmatic individuals. Differences in the pattern of fungi present in asthma patients and controls merit further investigation. Of particular interest was the presence of Malassezia pachydermatis, which is known to be associated with atopic dermatitis. Keywords: Asthma, Sputum, Fungi, Case ontrol studyBackground The human lung has a surface area of around 50 m2 [1] and is in contact with more than 15,000 litres of air each day [2].Ohorts. Clin Infect Dis 2012, 54:167?74. 34. Hospenthal DR, Murray CK, Andersen RC, et al: Guidelines for the prevention of infections associated with combat-related injuries: 2011 update. J Trauma supplement 2011, 71:S210 234.doi:10.1186/1471-2334-13-68 Cite this article as: Vento et al.: Multidrug-resistant gram-negative bacteria colonization of healthy US military personnel in the US and Afghanistan. BMC Infectious Diseases 2013 13:68.Submit your next manuscript to BioMed Central and take full advantage of:?Convenient online submission ?Thorough peer review ?No space constraints or color figure charges ?Immediate publication on acceptance ?Inclusion in PubMed, CAS, Scopus and Google Scholar ?Research which is freely available for redistributionSubmit your manuscript at www.biomedcentral.com/submit
van Woerden et al. BMC Infectious Diseases 2013, 13:69 http://www.biomedcentral.com/1471-2334/13/RESEARCH ARTICLEOpen AccessDifferences in fungi present in induced sputum samples from asthma patients and non-atopic PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28666375 controls: a community based case control studyHugo Cornelis van Woerden1*, Clive Gregory1*, Richard Brown2, Julian Roberto Marchesi2, Bastiaan Hoogendoorn1 and Ian Price MatthewsAbstractBackground: There is emerging evidence for the presence of an extensive microbiota in human lungs.
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